ALZ PRECURS PROT A4; MSX-100UL

Code: MAB348-100UL D2-231

Analysis Note

ControlBrain tissue, brain cell lysates

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeurosci...


read more

Your Price
€784.00 100UL
€964.32 inc. VAT

Analysis Note

ControlBrain tissue, brain cell lysates

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunofluorescence

Immunohistochemistry: 5-10 µg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).

Western blot: reducing conditions, 10µg/ml.

Optimal working dilutions and protocols must be determined by the end user.

Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

Immunogen

Epitope: a.a. 66-81 of APP {N-terminus}

Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Specificity

Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

110/120/130 kDa

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone22C11, monoclonal
Gene Informationhuman ... APP(351)
isotypeIgG1
manufacturer/tradenameChemicon®
NCBI accession no.NM_201413.1, NM_201414.1, NM_000484.2
Quality Level100
shipped indry ice
species reactivityhuman, fish, canine, monkey, pig, mouse, rat
technique(s)western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunofluorescence: suitable
UniProt accession no.P05067
This product has met the following criteria to qualify for the following awards:



PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN


GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.