Analysis Note
ControlBrain tissue, brain cell lysates
Application
Research Sub CategoryNeurodegenerative Diseases
Research CategoryNeuroscience
Immunofluorescence
Immunohistochemistry: 5-10 µg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).
Western blot: reducing conditions, 10µg/ml.
Optimal working dilutions and protocols must be determined by the end user.
Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.
Immunogen
Epitope: a.a. 66-81 of APP {N-terminus}
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Specificity
Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Target description
110/120/130 kDa
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